The reporter learned from Nankai University on the 28th that the State Key Laboratory of Medicinal Chemistry Biology led the research team to successfully "decipher" the two target genes of tetrodotoxin using monkey haploid neural stem cells and pass the gene knockout test. Letting neuroblasts acquire tetrodotoxin resistance, this study will make puffer fish poisoning "medicine rescueable", and related results are published online in the latest issue of the stem cell and neurodevelopment field "Development". The puffer fish is delicious, but the tetrodotoxin is extremely poisonous, often causing the ingestion to die instantly, and its target and toxicological mechanism are not known so far. According to some studies, tetrodotoxin is a kind of neurotoxin, and its toxicology may play a toxic role by blocking the sodium and potassium channels in nerve cells. So, can monkey haploid neural stem cells be used to screen the "effect point" of tetrodotoxin on the nervous system? Leading the team in the opposite direction, with the emerging haplocyte tool culture technology in recent years, by adopting the initial pluripotent culture system, the monkey haploid embryonic stem cells have better haploid maintenance and in vitro differentiation. Cell viability, and for the first time in the world, monkey haploid neural stem cells with in vitro proliferative capacity were obtained. In the experiment, the researchers immediately performed high-throughput whole-genome random mutations on the monkey haploid neural stem cells obtained, and the mutant cell lines were subjected to tetrodotoxin poisoning experiments. Finally, bioinformatics analysis and gene editing experiments confirmed that the key target genes of tetrodotoxin are B4GALT6 and SCN5A. From the genetic point of view, since the functional genes that specifically perform toxic functions are directly found, in theory, as long as these target genes are knocked out, the neuronal cells can obtain the resistance of tetrodotoxin. According to reports, the genetic similarity between primates and humans such as monkeys is very high. For humans, finding a target gene can be detoxified by taking a gene inhibitor. This also means that in the near future, people who have been infected with tetrodotoxin will no longer be incurable. (Technology Daily reporter Sun Yusong correspondent Wu Junhui) Ventilation is one of the most important components in a successful greenhouse. Greenhouse Circulation Fan,Greenhouse Ventilation Fan,Automated Greenhouse Ventilation Fan JIANGSU SKYPLAN GREENHOUSE TECHNOLOGY CO.,LTD , https://www.engreenhouse.com
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