In vivo injection of mouse tail vein injection technique

1. Immobilization of mice

It is best to use a mouse holder with a stomata at the front to ensure that the mouse breathes, and the tail can pull the tail out. Do not allow the mouse to have too much space in the holder. For example, if the space is large, some fillers may be added. Prevent mice from tampering during injection.

2. Preparation of the tail vein

The tail of the mouse can be bathed in warm water at about 50 ° C for 2 minutes to dilate the vein.

3. Selection of tail vein

There are 3 tail veins in the tail of the mouse. 1 day on the back and 1 on each side. Since the back vein is deep and thin, two sides are generally selected.

4. Syringe selection

Choose a 1-2 ml syringe with a 4 or 4.5 needle. A vaccinated disposable 2 ml syringe is preferred. After the syringe is inhaled, the air should be pushed out to prevent the death of the mouse due to air entering the vein.

5. Injection

After disinfecting the rat tail with 75% alcohol, the needle is usually inserted at 1/3 to 1/2 of the distal end of the tail. Here, the skin is thinner, the blood vessels are clear, the needle is easy to be inserted, and once the injection is unsuccessful, there may be more near There is room for end injection. The tail of the mouse to be injected is pressed tightly on the table with the left hand. When the needle is inserted into the right hand, the needle is parallel to the table top, and the needle tip is slightly downward. Once it is entered, the needle is slightly picked up, the needle enters along the blood vessel, and the needle can be seen by the naked eye. go ahead. If the needle advances in the blood vessel, it is noticeable that the needle is unobstructed and there is no resistance. If the needle is not in the blood vessel, there is resistance to the needle. Do not insert the needle too deeply. Immediately after the needle enters the skin, the needle is slightly upward, and the needle is inserted in parallel. When the needle is inserted, there is a feeling of falling. When there is no resistance when pushing the liquid, it indicates success. If the pushing resistance is large, and even if there is exudation at the injection site, it means that it is not in the vein and the injection needs to be re-adjusted.

6. Characteristics of successful injection

The injection of the drug into the vein and the non-venous difference, the injection into the vein has almost no resistance, can quickly push the liquid, usually 600ul can be pushed in 10 seconds. If the injection is outside the vein, the force is forcibly pushed, the resistance is large, and the dew-like exudate will appear around the injection site, and the bleeding will be less after the needle is removed. And because the drug solution is blocked in the tail, resulting in poor blood flow, tail ischemia, easily lead to inflammation and necrosis of the tail.

7. Wound treatment after injection is completed

Press the needle mouth with gauze until it does not bleed, usually 5-10 minutes. This will help the wound to recover as soon as possible to facilitate the next injection.
The article is from Ingen Bio's official website https://

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