High Efficiency Keratinase Enzyme for Feed Additive

 

It is not only an alkaline protease, but also can break down disulfide bond, disintegrate keratin completely. So reductase of disulfide bonds in the keratinase is the key enzyme in the course of keratinolysis.

 

Advantage1.A specific alkaline serine protease2.Specialized in degrading keratin3.Activity center includes serine4.A compound protease including reductase of disulfide bonds and polypeptide hydrolysis enzyme

 

Definition of content

One unit (U) of keratinase activity is defined as the amount of enzyme which liberates 1 μg tyrosine per minute from substrate casein at PH 10.5 and 40ºC. (GB/T 23527-2009)

 

Specification

 

Function of protease

1.Supplement the deficiency of endo-protease for animal.

2.Increase protein digestibility in animal feed and reduce the dosage of protein in feed formula.

3.Decrease the difference of feedstuff from different area and stabilize production performance.

4.Protect the environment and lessen pollution from nitrogen.

5.Develop out some new feedstuff e.g hydrolyzing feather meal, soybean meal and blood plasma powder.

 

Particular function of Keratinase

1. Degrade powerfully some anti-nutritional factors to be not broken down by trypsin and chymotrypsin e.g trypsin inhibitor, agglutinin etc.

2.Increase hydrolyzing degree of protein and proportion of soluble small protein in animal feed, which is conducive to animals' absorption, e.g rise hydrolyzing degree 20% for corn protein, 10% for meat and bone meal, 5% for feather meal and soybean meal

3.Improve the digestibility of amino acid, especially for methionine, histidine and serine, keratinase have better action.

 

 

Applications (Adjustable according to experiment results)

1.For leather processing, it can shorten processing time, simplify working procedure and improve product quality. Recommended dosage of 100000 U/g protease is 0.02-0.1%, 20-25ºC, 12-20h for retting process; 0.05-0.2%, 35-38ºC, 3-6h for leather soften; 0.1-0.3%, 20-35ºC, 12-20h for leather depilation.

2.For silk degumming, it may make silk lint-free and fluffy without damaging silk. After pretreatment, the recommended dosage of 100000 U/g protease is 0.8-2.4% pH9-11, 40-50ºC, 30-60min.

3.For chondroitin production, it can improve yield and purity, after extracting alkali, recommended dosage of 100000 U/g protease is 0.2-0.6%, pH8-10, 40-50ºC, 4-8h.

4.For heparin sodium production, it can increase uniformity of molecule and purity, after salt solution, recommended dosage of 100000 U/g protease is 2-4g per small intestine,pH9-11, 45-60ºC, 4-6h.

5.For detergent production, it can remove the stains of sweat, blood, protein and milk, and get a unique detergent effect. Recommended dosage is 0.1-1.0%

6.For animal feed, it can break down protein completely and remarkable raise the availability of feed protein. Recommended dosage of 50000 U/g is 20-25g/t in complete feed

 

 

It is not only an alkaline protease, but also can break down disulfide bond, disintegrate keratin completely. So reductase of disulfide bonds in the keratinase is the key enzyme in the course of keratinolysis.

 

Advantage1.A specific alkaline serine protease2.Specialized in degrading keratin3.Activity center includes serine4.A compound protease including reductase of disulfide bonds and polypeptide hydrolysis enzyme

 

Definition of content

One unit (U) of keratinase activity is defined as the amount of enzyme which liberates 1 μg tyrosine per minute from substrate casein at PH 10.5 and 40ºC. (GB/T 23527-2009)

 

Specification

 

Function of protease

1.Supplement the deficiency of endo-protease for animal.

2.Increase protein digestibility in animal feed and reduce the dosage of protein in feed formula.

3.Decrease the difference of feedstuff from different area and stabilize production performance.

4.Protect the environment and lessen pollution from nitrogen.

5.Develop out some new feedstuff e.g hydrolyzing feather meal, soybean meal and blood plasma powder.

 

Particular function of Keratinase

1. Degrade powerfully some anti-nutritional factors to be not broken down by trypsin and chymotrypsin e.g trypsin inhibitor, agglutinin etc.

2.Increase hydrolyzing degree of protein and proportion of soluble small protein in animal feed, which is conducive to animals' absorption, e.g rise hydrolyzing degree 20% for corn protein, 10% for meat and bone meal, 5% for feather meal and soybean meal

3.Improve the digestibility of amino acid, especially for methionine, histidine and serine, keratinase have better action.

 

 

Applications (Adjustable according to experiment results)

1.For leather processing, it can shorten processing time, simplify working procedure and improve product quality. Recommended dosage of 100000 U/g protease is 0.02-0.1%, 20-25ºC, 12-20h for retting process; 0.05-0.2%, 35-38ºC, 3-6h for leather soften; 0.1-0.3%, 20-35ºC, 12-20h for leather depilation.

2.For silk degumming, it may make silk lint-free and fluffy without damaging silk. After pretreatment, the recommended dosage of 100000 U/g protease is 0.8-2.4% pH9-11, 40-50ºC, 30-60min.

3.For chondroitin production, it can improve yield and purity, after extracting alkali, recommended dosage of 100000 U/g protease is 0.2-0.6%, pH8-10, 40-50ºC, 4-8h.

4.For heparin sodium production, it can increase uniformity of molecule and purity, after salt solution, recommended dosage of 100000 U/g protease is 2-4g per small intestine,pH9-11, 45-60ºC, 4-6h.

5.For detergent production, it can remove the stains of sweat, blood, protein and milk, and get a unique detergent effect. Recommended dosage is 0.1-1.0%

6.For animal feed, it can break down protein completely and remarkable raise the availability of feed protein. Recommended dosage of 50000 U/g is 20-25g/t in complete feed