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The FluorCam chlorophyll fluorescence system and hand-held fluorometers of the European PSI company, which is exclusively represented by Beijing Yiketai Ecological Technology Co., Ltd., have been recognized and used by research institutes such as universities and agricultural academies in China, and the Institute of Botany of the Chinese Academy of Sciences. The top units such as China Agricultural University cooperated and exchanged. At the same time, more than 500 papers have been published at home and abroad. This article introduces the literature published by domestic users in the near future.
1 College of Corn Seedlings of Shanxi University, Glyphosate and Cadmium Stress Researchers at the College of Environment and Resources, Shanxi University, conducted a combined stress of glyphosate (PMG) and heavy metal cadmium (Cd) on maize seedlings, with a single PMG stress and two PMG and Cd stresses. In the series, each series was set to control and 5 different concentrations of PMG treatment. The PMG concentrations of single and complex stress were set to 0, 1.25, 2.5, 5, 10, 20 mg. Kg -1 , Cd 2 in combined stress. The concentration settings were all 5 mg.kg -1 , and the fluorescence kinetic curves and parameters were detected by FluorPen 100 chlorophyll fluorescence spectrometer ( Chen Jiayue et al. Effects of glyphosate and cadmium combined stress on antioxidant enzyme activities and photosynthesis in maize seedlings Journal of Ecotoxicology, 2018, 13(1): 219-228 ). 
Researchers at Northwest A&F University used wild type (WT) and gene mutant ( var5-1 ) Arabidopsis thaliana as test materials to detect leaf reticular structure and chlorophyll fluorescence parameters of Arabidopsis thaliana plants using FluorCam open chlorophyll fluorescence imaging system . And changes in the image. ( Liang et al. Mutations in the Arabidopsis AtMRS2-11/AtMGT10/VAR5 Gene Cause Leaf Reticulation. Frontiers in Plant Science : 2017.02007 ) 
1. Shang-GuanK, et al . 2018. Lipopolysaccharides trigger two successive bursts of reactive oxygen species at distinct cellular locations. Plant Physiology, DOI: 10.1104/pp.17.01637. (Zhejiang University)
2. Zhao L, et al . 2018. Enzymatic activity and chlorophyll fluorescence imaging of maize seedlings ( Zea mays L.) after exposure to low doses of chlorsulfuron and cadmium. Journal of Integrative Agriculture: 16(0),60345-7. Shanxi University)
3. Cheng Xiangfei et al., 2018. Changes of chlorophyll fluorescence and antioxidant enzyme activities in chrysanthemum leaves under low temperature stress. Henan Agricultural Sciences, 47(4): 104-108. (Henan Agricultural University)
4. LiuT, et al . 2018. H2O2 mediates ALA-induced glutathione and ascorbate accumulation in the perception and resistance to oxidative stress in Solanum lycopersicum at low temperatures. BMC Plant Biology, 18(34), https://doi.org/ 10.1186/s12870-018-1254-0. (Northwest A&F University)
5. WangL, et al . 2017. The Phytol Phosphorylation Pathway Is Essential for the Biosynthesis of
Phylloquinone, which Is Required for Photosystem I Stability in Arabidopsis . Molecular Plant, 10: 183-196. (Institute of Botany, Chinese Academy of Sciences)
6. Yang Z, et al . 2017. RNase H1 Cooperates with DNA Gyrases to Restrict R-loops and Maintain Genome Integrity in Arabidopsis Chloroplasts. The Plant Cell, https://doi.org/10.115/tpc.17.00305.(Tsinghua University )
7. GongB, et al . 2017. Hydrogen peroxide produced by NADPH oxidase: a novel downstream signaling pathway in melatonin-induced stress tolerance in Solanum lycopersicum . Physiologia Plantarum, 160(4): 359-479. (Shandong Agricultural University)
8. Sui X, et al . 2017. The complex character of photosynthesis in cucumber fruit. Journal of Experimental Botany, 68(7): 1625-1637. (China Agricultural University)
9. Zhou C, et al . 2017. Bacillus amyloliquefaciens SAY09 increases cadmium resistance in plants by activation of auxin-mediated signaling pathways. Genes, 8(7): 173. (Tongji University)
10. Wei Xiaotong et al. 2017. Physiological responses of different allelopathic Chinese fir clones to continuous planting. Journal of Forests and Environment, 37(1): 22-28. (Fujian Agriculture and Forestry University)
11. Zhang L, et al . 2016. VIPP1 Has a Disordered C-Terminal TailNecessary for Protecting Photosynthetic Membranes against Stress. Plant Physiology, 171: 1983-1995. (Inner Mongolia University of Science and Technology)
12. Zheng C, et al . 2016. Global Transcriptional AnalysisReveals the Complex Relationshipbetween Tea Quality, Leaf Senescence and the Responses to Cold-DroughtCombined Stress in Camellia sinensis . Front. Plant Sci. 7, 1858.doi: 10.3389/fpls.2016.01858. Qingdao Agricultural University)
13. Huang S, et al . 2016. Physiological Characterization and Comparative Transcriptome Analysisof a Slow-GrowingReduced-Thylakoid Mutant of Chinese Cabbage ( Brassicacampestris ssp. pekinensis). Front. Plant Sci: 7, 3. (Shenyang Agricultural University)
14. Kong LA, et al . 2016. Comparison of the photosynthetic characteristics in the pericarp and flag leaves during wheat ( Triticum aestivum L.) caryopsis development. Photosynthetica, 54(1): 40-46. (Shandong Academy of Agricultural Sciences)
15. Wang LS, et al . 2016. Singlet oxygen-and EXECUTER1-mediated signaling isinitiated in grana margins and depends on theprotease FtsH2. PNAS, DOI:10.1073/pnas.1603562113. (Shanghai Institute of Plant Science, Chinese Academy of Sciences)
Yi Ketai chlorophyll fluorescence technology domestic application case
Chlorophyll fluorescence is sensitive, rapid and non-invasive to plants, and is a sensitive probe for studying plant photosynthesis. Chlorophyll fluorescence is widely used in plant science such as plant stress, disease detection, phenotypic research, and mutant detection. 2 Henan Agricultural University Chrysanthemum leaves low temperature stress Researchers at Henan Agricultural University used chrysanthemum leaves as test materials for low temperature stress. There were 5 temperature treatments: 16, 8, 4, -4, -8 °C (16 °C is suitable for chrysanthemum growth) Temperature), treatment at different temperature conditions for 3, 6, 9, 12h , using FluorCam portable fluorescence imager to detect changes in chlorophyll fluorescence parameters ( Cheng Xiangfei et al., chlorophyll fluorescence characteristics and antioxidant enzyme activities in chrysanthemum leaves under low temperature stress. Henan Agriculture Science, 2018, 47(4): 104-108 ) .
The above figure shows the effect of single PMG and Cd stress on the rapid chlorophyll fluorescence induction kinetics curve (OJIP) of maize leaves.
 The following table shows the effects of single PMG and Cd stress on chlorophyll fluorescence parameters of maize leaves.
The above figure shows the effect of low temperature stress on the chlorophyll fluorescence parameters of chrysanthemum leaves.
3 Different light quality treatments of Schima superba and Chinese fir seedlings in Fujian Agriculture and Forestry University The Forestry College of Fujian Agriculture and Forestry University used the southern tree species Schima superba and Chinese fir seedlings as experimental materials, and used three-color light plant incubators to carry out red light (662 nm) and blue light (460 nm) Color light treatment, with white light (446 nm) as a control. All the seedlings were treated with different light quality treatments for 30 days, and the chlorophyll fluorescence parameters were measured by FluorCam chlorophyll fluorescence imager ( Liu Qingqing et al. The effects of different light qualities on the chlorophyll fluorescence parameters and antioxidant enzyme activities of Schima superba and Cunninghamia lanceolata leaves. Journal of Ecology , 2018, 37(3): 869-876 ). The above figure shows the effects of different light quality treatments on chlorophyll fluorescence parameters (Fo, Fm, Fv and Ft) in leaves of Schima superba and Cunninghamia lanceolata.
4 citrus Zhejiang University, Zhejiang University blade greening disease of citrus leaves researchers to test material, FluorCam closed chlorophyll fluorescence imaging cytometry health, Huanglongbing (HLB) infections, nutritional deficiencies citrus leaf chlorophyll fluorescence characteristics (Cen et al. Chlorophyll Fluorescence Imaging Uncovers Photosynthetic Fingerprint of Citrus Huanglongbing . Frontiers in Plant Science , 2017: ( 8 ) 1509 ). The above figure shows the chlorophyll fluorescence parameters of three different treatments of citrus leaves.
The above figure shows the chlorophyll fluorescence parameters of three different treatments of citrus leaves.
5 Northwestern A&F University Arabidopsis Mutant Detection The above image shows the Fv/Fm parameters of WT and var5-1 Arabidopsis thaliana and its true leaves.
The above image shows the WT, var5-1 and var5-1 complements ( var5-1 P35S: At5g22830) Arabidopsis plants and true leaf Fv/Fm parameters.
Some domestic users publish bibliographies