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Feto Protein Staining Buffer
product description:
The Coomassie Brilliant Blue Protein Ultrasonic Staining Solution (Feto SDS PAGE) is a protein dye that can be dyed at room temperature without discoloration, non-toxic and non-irritating odor. This product is suitable for all kinds of denatured and non-denaturing protein gel dyeing, which can save a lot of time and accelerate the experiment process. With its convenient and quick operation, excellent sensitivity and green environmental protection, it has become the first choice among many protein dyeing products.
Instructions:
1. Remove the electrophoresed PAGE gel into a plastic container and add a suitable amount of dyeing solution to cover the PAGE gel. Depending on the size of the container, it is usually about 30-50 ml.
2. Shake on a shaker. Because the dye solution is acidic, do not touch the dye solution with bare hands. The band began to appear after 1-2 minutes. The dyed strips will deepen over time. Usually the dyeing time can be from 30min to overnight. The background color does not substantially increase with the dyeing time. The dye solution can be reused 3 times. As the number of uses increases, the dyeing speed decreases slightly, but does not affect the sensitivity.
3. After the dyeing is finished, the protein gel is stored in water and taken out for photographing. Due to the different isoelectric points of the protein, the dye has different instantaneous attachment ability. It is recommended to dye the gel for 2 hours or overnight, and then put the gel into the water for better effect.
Features:
Fast dyeing, only dyed protein is not dyed, no need to decolorize, especially for the experiment of protein purification, after a few minutes, the next step can be determined according to the results of protein staining. The whole process is easy to operate, easy to store, no special equipment is needed, and the gel can be stored in pure water for several months after decolorization.
Tip: The thickness of the glue exceeds 1mm. Please add more dye solution and extend the dyeing time.
Scope of application:
This product is suitable for SDS-PAGE glue and Native glue prepared by the laboratory itself or prefabricated. It can also be used after Western film transfer.
Staining of residual proteins on the PAGE gel.
Tip: Like the traditional Coomassie blue dye solution, the dye solution binds to the positively charged group on the protein. Quantitative comparison between different proteins requires consideration of the number of positively charged amino acids. This also explains that many of the proteins observed by users are easy to stain and some are lighter. Pro, this is related to the amount of lysine and arginine contained in the protein and the isoelectric point.
Storage Conditions:
Normal temperature can be stored for one year.
Feto SDS-PAGE staining solution instructions
Feto SDS-PAGE staining solution