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Pacific Oyster Artificial Seedling Technology
First, the pro-shellfish selection Parents with shellfish to 1 instar ~ 3 years old, shell length of 12 cm or more as well. The pro-scallops were picked during the harvest season and placed in cages for intensive intensification on dedicated trusses. When using pro-scallops, they should be selected within the natural discharge period of the oysters. The pro-strips should be taken from the sea area for direct oxy-production, or moved into a larger pool for storage in still water, so that they can continue to be used after the pro-Rex emissions from the sea area. To elicit labor. Second, the acquisition of sexual cells with anatomy and dry heat-induced water stimulation induced oxytocin available a large number of fertilized eggs, artificial breeding. Anatomical manipulations are performed according to normal practice. The process of stimulating the oxytocin to stimulate the oxytocin is to first wash the pro-shellfish clean and carefully remove all attached organisms from the shell surface. After drying for 12 hours (usually from 18 o'clock in the evening to 6 o'clock in the morning), it is then moved to the sun and covered with bamboo curtains. After heating, the duration of 10 hours or so, then the pro-shellfish into the cage, each cage pro-shell 15 to 20, suspended into the urine production pool, using a water pump at a rate of 65 tons / hour into the fresh seawater sedimentation Close to the wall when the valve is filled, so that the pool of water to keep flowing, stimulated by water for 2 hours, stop the water injection, to be pro-shell emissions. The effect of oxytocin was better when the pro-shellworm was in the 1st to 2nd day before labor. In addition, there is a direct relationship with the magnitude of warming. Third, the larvae cultivate pro-shellfish in the urine production pool after fertilization, when the embryo develops to the D-shaped larvae, the larvae are screened, that is, the larvae in the pool water layer 3/4 water, through the pool wall valve into the nursery pool cultivation, the remaining 1 /4 Discard all water bodies and residual eggs at the bottom. The density of D-shaped larvae was 15 to 20/ml. In the 3 days to 5 days from the start of culture, 20 cm to 30 cm of water was added daily, and when the individual larvae reached 100 microns or more, the siphon water changer NX103 was used for siphon water exchange, and the amount of water was changed by about 1/2 per day. Baits mainly use four kinds of Chaetoceros, Platymonas, Chlorella and X-Algae. According to different developmental stages of the larvae, the feeding type and feeding amount are generally: D. larvae is fed to the glacier or X-algae, and the daily feeding amount is 190,000 cells/ml to 200,000 cells/ml. In the larval stage of shell larvae, G. gambiense, Chaetoceros muelleri, and Platymonas are mixed and fed. The amount of daily feeding is G. genozoa 20,000 cells/ml, Chaetoceros 14,000 cells/ml to 24,000 cells/ml, Platycladi 0.3. Million cells/ml to 0.6 million cells/ml. The eye-point larvae fed Chaetoceros and Platymonas. The amount of daily feeding was Chaetomium 20,000 cells/ml to 40000 cells/ml, Platymonas subtilis 0.3 million cells/ml. In order to maintain the freshness of the cultured water, the reservoir will be replaced with fresh seawater once every two days, and the seawater used in the sediment will not exceed 3 days. During the nursery period, the physical and chemical factors of the water in the nursery pond generally range from 25°C to 30°C, a specific gravity of 1.01 to 1.0818, a pH of 8.0 to 8.4, and a dissolved oxygen of 4 mg/L to 6 mg/L. Fourth, with the seedlings and seedlings larvae developed to 280 microns to 300 microns, when the emergence of eye spots and feet began to put seedlings with seedlings, seedlings with shrimp scallop shell, oysters and cement strips better. The shells are strung together with 60 pieces of polyethylene rope and are about 60 centimeters in length. Although rubber strips work well in the sea area, the effect of putting seedlings in nursery ponds is poor. Before harvesting the seedlings, reduce the water to about 70 cm. Then, attach the seedlings to the pool and hang it in the pool. With a distance of 10 cm between the seedlings, the method of continuously raising and lowering the water level and turning the upper and lower positions of the seedlings upside down in order to attach seedlings evenly and reduce the difference in the amount of yin and yang attachment. For the artificial breeding of Pacific oysters, seedlings can be bred from small indoor water bodies (below 5 cubic meters of water) to large outdoor water bodies (above 50 cubic meters water level), and the effects of outdoor large-water sports seedlings are relatively stable, and the period of seedlings can be shortened. Simplifying the seedling process is very convenient. The bred juveniles are held in the pond until the late 10th and then moved to the sea area for cultivation, so as to prevent the juveniles from falling into the sea to be stocked by the barnacles and causing losses.