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High-yield Cultivation Techniques for Tuber in vitro
Musa is one of the main varieties of bananas and is native to Southeast Asia. Generally 1500-2000 kg per mu, up to 2500-3000 kg. The banana fruit is unique in flavor and rich in many trace elements and vitamins. Vitamin C content is close to those of mango, pineapple, orange and other fruits. The banana is resistant to cold and can be processed into banana slices to increase added value. In recent years, the price of bananas has been higher than Taiwan bananas, and it is worth promoting. Test tube seedlings usually have the advantages of no pests and diseases, uniform growth, and easy transportation, which is favorable to the large-scale promotion of improved varieties, and is currently the commonly used seedlings of American bananas. However, the test-tube seedlings are tender and weak at seedling stage, and have poor resistance. They are prone to mosaic heart rot and other diseases and pests, and are easily damaged or planted in bad weather. The production period is also longer, and the tissue culture process is prone to variation. Part of the mutation is still difficult to identify when planting. For these characteristics, the management of planting will be summarized as follows: 1. Site preparation and soil cultivation. In vitro plantlets are prone to outcrop, and they are not suitable for deep-seeds. It is best to leave enough soil for soil afterwards. The Shuitian Tea Garden is suitable for three-stage tricks, that is, 10 to 15 cm above the planting hole. As the test-tube seedling grows, the soil in the cocoon will be planted in the planting hole to prevent the outcrop. During the field preparation, the ditch can be shallow for a short time. Afterwards, digging deep ditch and digging the soil to the plants. Second, planting. Test tube seedlings 6-8 leaves (including bottle seedlings 3 leaves) can be planted, the general spring planting is good. Spring plants should be selected for warm and windless weather, and summer and autumn plants should be planted on cloudy days in the afternoon. Before planting, plant the seedlings in advance to allow the seedlings to adapt to the natural climate. Be careful when opening the seedling bags when planting. Do not loosen the bag soil, otherwise it will affect the survival rate and restore growth. Third, fertilization. The test tube seedlings are not very fertile at the initial stage, and the basal fertilizer must be deeply applied. The root system must not touch the loam, and the farmyard fertilizer must be rotted and deep-applied to a depth of more than 30 cm or applied to a depth of 60-80 cm. The seedlings can be cleared in the rain and collected after planting. When new leaves are topdressing. Within two months after planting, 0.1%-0.2% compound fertilizer or potassium dihydrogen phosphate solution can be used to accelerate the harvest of 5,000 times of liquid fertility in dry days, and 1-2 kilograms of fertilizer solution can also be used. In the rain, about 10 grams of urea or compound fertilizer can be sprinkled 15-20 centimeters away from the seedlings, applied every 7-10 days, and applied once, and conditions can be combined with the application of plant nutrient plus potassium dihydrogen phosphate, organic fertilizer solution. , fast harvest, Gao Lida, Ye Bao, etc., With the growth of plants, the amount of leaching or fertilization can be larger. The amount of medium-term fertilization should be large, once in 15-20 days, and once in the late 25-30 days. Spring planting seedlings need to strengthen fertilizer management in order to catch buds in winter. The well-watered plantlets grew vigorously, and the new canopy leaves were spirally curved, one larger than the other. Fourth, water management. In the initial stage, the test tube seedling needs less water, but it can not be drought and soil waterlogging. Drought should be timely watering, planting can be covered with planting holes in the high temperature period, the rainy season must pay attention to soil drainage, sticky soil can be planted 30-40 cm outside the planting hole, let the planting hole shoot 5 - 10 cm to prevent water from burying the cavities and causing rot or root damage. Fifth, strengthen pest control. Mosaic heart rot disease is the main disease of test tube seedlings. The prevention and treatment of the disease can be used well-resistant old strong test tube seedlings, timely removal of weeds in the field, not the kind of virus host crops, spray anti-virus agents regularly for prevention and treatment. Seedlings of test-tube seedlings are also susceptible to pests such as the larvae of the beetle moth. Attention should be paid to the control of spraying. DuPont can hit 155 times the suspension agent insecticide 3500 times or DuPont Wanling 90% soluble powder plus DuPont Fuxing 40% 8000 times. Liquid or DuPont eBao 68.75% dispersible granules 1000 times spray, control effect is very good. Sixth, timely removal of mutant strains. Variants in the nursery period, such as leaves, streaks, mosaics, and deformed leaves, are more easily identified as extra-dwarfed seedlings. In addition, there are two variants that are more variable and are initially difficult to recognize. They are usually recognizable after 2-4 months of colonization and 15-20 days of age, one of which is dwarfing and the other is It is an abnormal leaf. These two major variants can be buds and bear fruit, but the yield is extremely low, and the quality is poor. Most of them have no economic value and must be discovered and excavated as soon as possible and replanted in a timely manner. In general, 5% of the strains are allowed according to the regulations. Usually, 10% more seedlings should be purchased, and 15 cm 15 cm 15 cm large bags or directly in the field should be planted to eliminate diseased strains, mutant strains and dead strains. Replanting. Seven, in addition to buds. The plants planted by the test-tube seedlings were soaked and sucked earlier, and they were numerous but weak. Due to the late determination of leave buds, these early pumping buds should be removed as soon as possible, and can be removed with a special sharp banana or sickle when the buds are 15-20 cm long. (Huang Defa)