During the production of edible fungi, it is often exposed to mold, such as Trichoderma and Aspergillus. These bacteria not only compete with the edible fungi for nutrients and space, but also secrete various toxins to inhibit the growth of mycelia and fruit body development of the edible fungi, resulting in reduced production and quality. At present, there are few kinds of fungicides available for the production of edible fungi. For this reason, I chose 6 kinds of biological fungicides that have good control effect on plant diseases, such as mycin and Nanning ethamcin, to study their effects on Pleurotus ostreatus and other fungi. The effect of mycelial growth is to screen out fungicides that can effectively control mold contamination without affecting the normal growth and development of edible fungi. The six biocides tested were 3% enzymatic wettable powder, 8% nannanmycin, 5% Jinggangmycin, 6% kasugamycin wettable powder, and 1.5% multi-resistance. Wettable powders and 4% pyrimidine nucleoside antibiotics. After diluted 1000-fold each time, the effect of mycelial growth inhibition rate on the growth of Trichoderma, Aspergillus flavus, Aspergillus niger, and Pleurotus ostreatus were measured using a plate mycelium growth inhibition assay. The experimental data show that the above 6 kinds of bio-fungicides have certain inhibitory effects on the three kinds of bacteria and mushroom mycelia. Among them, the inhibition of mycelin on the three mycelial hyphae was the largest, followed by Ningnanmycin. The two drugs had the least inhibitory effect on Pleurotus ostreatus mycelium. Tests have shown that Zhongshengmycin and Ningnanmycin are suitable for use in Pleurotus ostreatus production. Dongguan Anmeixuan Electronic Technology Co., Ltd , https://www.hyashine.com