Ding Yiyuan TL1000 automatic grinder grinding mouse skin extraction RNA experimental steps

Nankai University teaches you to use the Dingyuanyuan TL1000 automatic grinder to grind mouse skin to extract RNA.

Experimental location: Nankai University

Test sample: mouse skin (RNA extraction)

Experimental steps:

1, in a 2ml round bottom centrifuge tube single tube into the mouse skin sample 40-50mg;

2. Add 1mm hard stainless steel grinding beads to the centrifuge tube, cover the tube cover, and load it into the Dingyuan TL1000 automatic grinder adapter;

3. Immerse the adapter containing the sample in liquid nitrogen;

4. After the liquid nitrogen is not boiled, it is taken out and quickly installed on the machine, using intermittent grinding method;

5, intermittent grinding parameters set to 2000rpm, each automatic grinding 1 minute, intermittent 10s, cycle 4-5 times the best effect, from the gel map to see higher RNA concentration;

6. After the completion of the grinding, the centrifuge tube was taken out, and 0.5 ml of the lysate (Trizol) was added to each tube, followed by centrifugation, purification, etc., for subsequent RNA extraction experiments.

Experimental experience:

According to the user's experimental experience, the mouse skin was ground using the Dingyuan TL1000 automatic grinder. The parameter setting speed was greater than 1600 rpm. Other settings were set in the above cases, and the RNA could be successfully ground and extracted. However, the automatic grinding effect is best with the method in the case.

YT-60

YT-60

YT-60

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