PCR ( Polymerase Chain Reaction)
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It provides a way to make more copies of a portion of DNA.
We need to have PCR ingredients that include DNA portion, Buffer solution, Primer+DNA (tap polymerase), and Nucleotides during the preparation step.
In the step of PCR sequence, there are three steps in this section.
Step 1 Denaturation
The addition of heat is needed to separate the two strands of the DNA molecule.
Step 2 Annealing
Temperature, is going to be cool in this step, should allow the primers to bind to the specific segments.
Step 3 DNA Synthesis
To make more copies of the DNA.
Those three steps above keep repeating to make enough copies of the DNA fragments. It is the principle of how PCR machine works.
Multi-function extraction tank extraction operation - Xiangpai Machinery
Multi-function extraction tank extraction operation - Xiangpai Machinery
(1) Forced circulation In the extraction process, for the extraction rate, the pump can be used for forced circulation (but not for drugs containing more starch and more viscous). The forced circulation means that the liquid medicine is discharged from the liquid discharge port at the lower part of the tank, filtered through the pipe filter, and then returned to the tank body with a water pump. The method accelerates the relative motion between the solid and liquid phases, thereby enhancing the convection diffusion and leaching process and improving the leaching efficiency.
(2) After the extraction and extraction of the extract is completed, the extract is discharged from the lower liquid discharge port of the tank, filtered through a pipe filter, and then pumped to the concentration section for concentration.
(3) Recirculation cycle During the extraction process, a large amount of steam is inevitably generated in the tank. The steam enters the heat exchanger through the foam trap for condensation, enters the cooler for cooling, and then enters the gas-liquid separator for gas-liquid separation. The residual gas escapes and the liquid flows back into the extraction tank. This cycle until the extraction is terminated.
(4) If the heating method is water extraction, after the water and Chinese medicine are put into the extraction tank, the steam is introduced into the tank for direct heating; when the temperature reaches the temperature of the extraction process, the steam is stopped in the tank, and the interlayer is changed. Steam is used for indirect heating to maintain the temperature inside the tank within a specified range. In the case of alcohol extraction, all of the indirect heating is carried out by means of a layer of steam.
(5) Extraction of volatile oil When performing general water extraction or alcohol extraction, the valve leading to the oil-water separator must be closed, and only when volatile oil is present. The heating method is basically similar to the water extraction operation. The difference is that the liquid medicine vapor in the extraction process is not cooled directly into the gas-liquid separator after being cooled by the cooler. At this time, the valve passage of the cooler and the gas-liquid separator must be closed. , and enter the oil-water separator to separate the oil and water, so that the required oil is discharged from the oil outlet of the oil-water separator; the aromatic water is separated from the return water pipe by the gas-liquid separator, the non-condensable gas is put into the atmosphere, and the liquid is returned to the air. Inside the can. The two oil-water separators can work alternately in turn, and the volatile oil is extracted. Some of the liquid that remains in the oil-water separator and cannot be returned can be discharged from the bottom drain valve.
Finally, Xiangxiang told everyone to develop a new extraction process, re-engineering the extraction equipment, so that the extraction tank of Chinese medicine has a short extraction time and high extraction efficiency. The extraction process conditions are the basic requirements for the extraction equipment. If you don't understand, you can ask the technical department of Xiangpai.
Xiangpai Machinery has been engaged in food machinery design experience for many years, focusing on earnest and sincere gratitude, creating quality products, pursuing customer satisfaction and adhering to the supremacy of credibility. Xiangpai Machinery welcomes your cooperation.