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Choose a plastic bag tube milk white translucent low pressure polyethylene plastic material, cut it into 35 cm long bag tube. The bag shouldn't be too wide, otherwise it will breed bacteria.
The formula for formulating culture materials is: 99% cotton husk, 1% bran or corn flour. To reduce bacterial contamination, 0.05% carbendazim can be added during the culture. The ratio of feed to water is 1:1.4 to 1:1.6. Mix well after adding enough water. After mixing, heap stuffy for 1 to 2 hours.
Bagging, Sterilization and Inoculation Take out the pre-prepared plastic bag tube, tie the end of the tube with a string, and then load the tube with 18 to 20 centimeters high, and then tie the tube mouth with a string to become two closed. Bags. It is sterilized at 125°C for 2 hours and can also be sterilized at 100°C for 8 hours. After the sterilization, the bag is moved into the inoculation room while it is hot, and the inoculation is performed after it is cool. When vaccinating, separate the two types of accession strains of the bag, and then use a string to tie the bag, the inoculation volume is 5%.
Bacteria culture After inoculation, the inoculated bags are stacked on the bed frame or on the ground in a “well†shape. The culture temperature is preferably kept at about 23°C, not lower than 18°C, indoor air relative humidity is about 60%, and the light should be dark.
After the fruiting management mycelium has covered the whole bag, see a small amount of mushroom buds can be timely transferred into the mushroom management. Set a mushroom shelf in the shed, and put the mouth of the mushroom bag outwards and layer it horizontally, stack 4 to 5 layers, and open the mouth of the bag to allow the fruit on both sides. During the mushrooming period, the temperature is maintained at about 10°C, the relative humidity of the air is 80% to 90%, and there must be moderate ventilation and light.
Bag-incorporating mushroom has high efficiency on both sides of mushroom
Flammulina edodes have accumulated and discharged on both sides of the mushroom bag, which has less investment, lower cost, and higher efficiency than the single-bag-discharged vertical side of the mushroom. Its technical points are as follows: