Annexin V/PI apoptosis kit (Cat#: AP101) is a kind of apoptosis of phosphatidylserine which appears on the surface of cell membrane when FITC-labeled recombinant human Annexin V is used to detect apoptosis. Detection kit. It can be detected using flow cytometry, fluorescence microscopy or other fluorescence detection equipment. The kit also provides propidium iodide (PI) staining solution. PI can stain necrotic cells or cells that lose cell membrane integrity in the late stage of apoptosis, showing red fluorescence. For necrotic cells, since the integrity of the cell membrane has been lost, Annexin V-FITC can enter the cytoplasm and bind to phosphatidylserine located inside the cell membrane, thereby also causing the necrotic cells to exhibit green fluorescence. Therefore, by matching Annexin V with PI, cells at different times can be distinguished. Kit components Catalog number / specification Component AP101-30 AP101-60 AP101-100 30T 60T 100T Annexin V-FITC 150ul 300ul 500ul 5x Binding Buffer 5ml 10ml 15ml Propidium Iodide (PI) 300ul 600ul 1000ul Sample processing (1) Inducing apoptosis by a suitable method; (2), suspension cells were centrifuged (1500 rpm for 5 min) to collect; adherent cells were digested with trypsin without EDTA and washed once with serum-containing medium (Note: trypsin digestion time is not too long, otherwise it is easy to cause false positive); Or directly with Accutase enzyme digestion (without termination); centrifugation at 1500 rpm for 5 min to collect cells; (3), washing the cells twice with PBS (centrifugation at 1500 rpm for 5 min) to collect 1-5 X105 cells, discard the supernatant; (4) Prepare 1X Binding Buffer buffer: Calculate the amount of 10 times, add 1ml of 5X Binding Buffer buffer to 4ml of deionized water; (5) Resuspend the cells with 500ul 1X Binding Buffer (buffer diluted in step 4) (6), add 5ul annexin-V FITC and 10ul PI (7) Incubate for 5 minutes at room temperature (8), machine detection Flow detection Flow analysis Annexin-V FITC (radiation wavelength = 488 nm; absorption wavelength = 530 nm) is detected by FITC signal (usually FL1 channel); PI dye solution is detected by PE signal (usually FL2 channel) Read the original text: http:// Skull Clamp,Neurosurgery Accessories,Operating Table Skull Clamp,Operating Bed Skull Clamp NINGBO TECHART MEDICAL EQUIPMENT CO.,LTD , https://www.techartmed.com Kit step