Instructions for use of plant chromatin immunoprecipitation kit

Product Name : Plant Chromatin Immunoprecipitation Kit, EpiQuik Plant ChIP Kit

Product number : P-2014

Product specifications : 24 reactions, 48 ​​reactions

Product introduction :

The EpiQuikTM Plant Chromatin Immunoprecipitation Kit includes a full suite of reagents that allow the tester to efficiently study protein-DNA relationships in vivo. The entire process can be completed in less than 6 hours, and the product is far superior to any competitor's kit. The EpiQuikTM Plant Chromatin Immunoprecipitation Kit is designed for use with specific immunoprecipitation in combination with qualitative and quantitative PCR, ChIP-Seq, and ChIP-on-chip.

This kit has the following advantages :

Fast and simple procedure, the experiment can be completed in 6 hours.

The form of the microplate makes the analysis flexible: manual or high throughput.

Includes DNA purification columns: saves time and reduces labor.

Compatible with all DNA amplification methods.

Simple, reliable and consistent detection conditions are achieved.

Background Information:

Protein-DNA interactions play a key role in cellular functions such as signal transduction, gene transcription, chromosome segregation, gene duplication and recombination, and epigenetic silencing. In plants, the interaction between DNA-binding proteins and homologous promoter sequences establishes temporal and spatial expression patterns of genes and affects balance, development and adaptability in vivo. Chromatin immunoprecipitation (ChIP) provides an advantageous tool for directly identifying the entire genome-wide association of specific regulatory proteins and their target genes. Unlike other methods such as EMASA, DNA microarrays, and reporter gene assays, which analyze DNA and protein interactions in vitro, chromatin immunoprecipitation (ChIP) can detect specific protein binding to a specific gene sequence in living cells.

program:

The kit includes all of the reagents required to successfully perform chromatin immunoprecipitation from plant cells. In particular, the kit includes a ChIP-grade dimethyl histone H3-K9 antibody and a negative control for normal mouse IgG. Extracted from cell chromatin, sheared, and added to antibody-closed microwells. After the antibody captures the protein-DNA complex, the DNA is released, inverted and purified by a specially designed rapid spin column. The eluted DNA can be used in a variety of downstream applications.

Starting material:

The starting material can include various plant tissues (flowers, leaves, seedlings). In general, each reaction input should be from 20 to 50 mg of plant tissue.