Human High Density Lipoprotein (HDL) ELISA Kit Instructions

Human High Density Lipoprotein (HDL) ELISA Kit

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Keshun Biological Reagents ranks among the best in the field of peers with superior production technology, high quality products and excellent service. There are a variety of product types such as ELISA kits, antibodies, biochemical reagents, standards/controls, serum, and various imported laboratory supplies. Welcome scientific research workers to come to consult! The company provides free testing services!

Free high-density lipoprotein (HDL) ELISA kit

Brand: Keshun Biological

Specification: 96T/48T

Substance service, full Elisa experimental technical guidance, free to do experiments.

Features: high sensitivity, specificity, good repeatability, short experimental period, strong biological activity, wide detection range, etc.

Wide range of applications: plasma, serum, tissue homogenate, cell culture supernatant, urine and other types of samples

The types of detectable animals are rich: human, monkey, rat, mouse, guinea pig, golden hamster, rabbit, pig, dog, cow, sheep, goat, goose, chicken, shrimp, river crab, squid, zebrafish, etc.

Common methods of ELISA: double antibody sandwich method, indirect method, competition method and BAS-ELISA.

Transportation: Off-the-shelf, generally for express delivery, Jiangsu, Zhejiang and Shanghai arrive every other day, and the field and remote places arrive in three to five days.

The human high-density lipoprotein (HDL) ELISA kit is a highly sensitive ELISA based on immunological reactions that combines the specific reaction of antigens and antibodies with the efficient catalysis of enzymes on substrates. Test technique.

Steps:

1. Remove the required slats from the foil pouch after equilibrating for 20 min at room temperature. The remaining slats are sealed back to 4 °C with a ziplock bag.

2. Set standard and sample wells, and add standard concentration of 50μL to each standard.

3. Add 50 μL of the sample to be tested to the sample well; blank holes are not added.

4. In addition to the blank wells, add 100 μL of horseradish peroxidase (HRP)-labeled detection antibody to each well of the standard wells and sample wells, seal the wells with a sealing membrane, and incubate in a 37 ° C water bath or incubator. 60min.

5. Discard the liquid, pat dry on the absorbent paper, fill each well with the washing solution (350 μL), let stand for 1 min, remove the washing solution, pat dry on the absorbent paper, and repeat the washing 5 times (can also be washed with a washing machine) board).

6. Add 50 μL of substrate A and B to each well and incubate at 37 ° C for 15 min in the dark.

7. Add 50 μL of stop solution to each well, and measure the OD value of each well at a wavelength of 450 nm within 15 min.

l Human high-density lipoprotein (HDL) ELISA kit common problem solving:

l Problem: Sample or standard OD is out of normal range

l Keshun technical answer: the dilution of the wrong sample or standard

l Solution: Dilute completely according to the instructions

l Problem: there is no obvious trend between the high concentration of the standard

l Keshun technical answer: only for single wavelength detection

l Solution:

l Because the reference wavelength reads non-specific detection, such as fingerprints, scratches, water stains, etc., it also affects the results. The final result is recommended to be the most accurate with dual wavelengths.

Human high-density lipoprotein (HDL) ELISA kit quality spot related products:

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The human high-density lipoprotein (HDL) ELISA kit is to be tested. The spot product will be sent to the customer within one week from the date of receipt of the customer's specimen! Years of professional services, quality assurance, escort your research, free of charge to provide testing services! In order to show the sincerity of cooperation, we also launched a free application for the trial, and provided technical guidance throughout the experiment, so that customers can better understand the quality and service quality of the company's products. For details, please contact our sales customer service, welcome your inquiry, request for instructions, inquiry message form, telephone, online customer service QQ, send email.

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