Identification of dried ginger

Identification

Character identification

The rhizomes are irregularly massive, slightly flat, with fingerlike branches, 3-7 cm long and 1-2 cm thick. Surface gray brown or light yellow brown, rough, with longitudinal wrinkles and obvious links. Branches often have scaly leaves remaining, branches with scars or shoots at the top. Solid quality, yellow-white or gray-white cross-section, powdery and granular, with a clear ring (endothelial layer), vascular veins (vascular bundles) and yellow oil scattered. It is fragrant, specific and spicy. It is better to have a solid quality, yellow-white cross-section, powdery feet, and strong smell.

Microscopic identification

Cross-section of rhizome: The wood column is composed of rows of flat wood cells. The cortex has the majority of leaf vascular bundles; the inner cortex is obvious and the Kjeldahl band is visible. The column occupies most of the rhizomes, and it has a majority of externally flexible vascular bundles. The vascular bundles in the near-to-sheath sheath are small and arranged closely. There are non-ligninized fiber bundles inside or around the xylem. This product has scattered oil cells in parenchyma. Parenchyma cells contain starch grains.

Physical and chemical identification

TLC: Divide 1g ginger, grind ginger 5g, add proper amount of methanol, shake and stand for 1h, filter. The filtrate was concentrated to approximately 1 ml and used as a test solution. The linalool and 1,8-oleophane were used as reference materials. The samples were spotted on the same silica gel G plate, using petroleum ether-ethyl acetate (85:15). ) Expand to 1% Vanillin Sulfuric Acid Liquor Color. The test solution chromatogram was in the same position as the reference chromatogram and showed the same spot.

Identification

(1) This product is light yellowish brown. Starch granules are many, oval, oval, triangular-oval, round or irregular shape, diameter 5 ~ 40μm, umbilical point-like, located in the smaller end, there are also crack-like person, layer patterns are obvious. The oil cells and resin cells are scattered in the parenchyma and contain light yellow oil droplets or dark reddish-brown substances. Fibres bundled or scattered, tip blunt, a few fork, some wavy or jagged, diameter 15 ~ 40μm, slightly thicker walls, non-wooden, with fine lines holes, often seen thin diaphragm. Ladder, thread and reticulate catheter are more common, a few are ring-shaped catheters, diameter of 15 ~ 70μm. Tubular cells containing dark reddish-brown material are sometimes seen next to the catheter or fiber and have a diameter of 12-20 μm.

(2) Take this product powder 2g, add ethanol 20ml, sonicated for 20 minutes, filtered, the filtrate evaporated, the residue was dissolved in methanol 1ml, as the test solution. Another ginger reference drug 2g, with the legal system as a reference drug solution. According to the thin layer chromatography (Appendix VI B) test, 4 μl of each of the above two solutions was pipetted and spotted on the same silica gel G-laminate plate with sodium carboxymethyl cellulose as the binder, with cyclohexane-ether (1) : 1) As a developing agent, unfold it, take it out, dry it, spray it with vanillin sulfuric acid test solution, and heat it at 105°C until the spot is clear. In the chromatogram of the test sample, spots of the same color were observed at the positions corresponding to the chromatogram of the reference drug.


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