Human isoproterenol ( iso-Hyd ) ELISA kit instruction manual This reagent is for research use only. Specimen: serum or plasma Fanke Biological Supply Purpose of use: This kit is used to determine the isoproterenol ( iso-Hyd ) content in human serum, plasma and related liquid samples . Test principle: The iso-Hyd kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA). Standards of known iso-Hyd concentration and samples of unknown concentration are added to the microplates for detection. The iso-Hyd and biotinylated antibodies were first incubated simultaneously. After washing, avidin-labeled HRP was added. After incubation and washing, the unbound enzyme conjugate is removed, then the substrates A, B, and the enzyme conjugate are added simultaneously. Produce color. The depth of the color is proportional to the concentration of iso-Hyd in the sample. Isoproterenol kit instructions elisa kit, ELISA kit, ELISA test kit, the kit instructions human elisa Kit content and its preparation Kit components (2-8 ° C) 96-well configuration 48 hole configuration Formulation 96/48 ELISA plate 1 board (96T) Half board (48T) Ready-to-use Plastic diaphragm cover 1 block Half block Ready-to-use Standard: 80ng/ml 1 bottle (0.6ml) 1 bottle (0.3ml) According to the instructions for thinning Blank control 1 bottle (1.0ml) 1 bottle (0.5ml) Ready-to-use Standard dilution buffer 1 bottle (5ml) 1 bottle (2.5ml) Ready-to-use Biotinylated anti-iso-Hyd antibody 1 bottle (6ml) 1 bottle (3.0ml) Ready-to-use Affinity chain enzyme-HRP 1 bottle (10ml) 1 bottle (5.0ml) Ready-to-use Washing buffer 1 bottle (20ml) 1 bottle (10ml) Dilute according to the instructions Substrate A 1 bottle (6.0ml) 1 bottle (3.0ml) Ready-to-use Substrate B 1 bottle (6.0ml) 1 bottle (3.0ml) Ready-to-use Stop solution 1 bottle (6.0ml) 1 bottle (3.0ml) Ready-to-use Specimen dilution 1 bottle (12ml) 1 bottle (6.0ml) Ready-to-use Self-contained materials 1. Distilled water. 2. Sampler: 5ul, 10ul, 50ul, 100ul, 200ul, 500ul, 1000ul. 3. Oscillators and magnetic stirrers, etc. safety 1. Avoid direct contact with the stop solution and substrates A, B. Once exposed to these liquids, rinse with water as soon as possible. 2. Do not eat, drink, smoke or use cosmetics during the experiment. 3. Do not use your mouth to take any ingredients from the kit. Operational precautions 1. Reagents should be stored according to the label instructions and returned to room temperature before use. Standards after dilution should be discarded and cannot be stored. 2. The slats not used in the experiment should be immediately put back into the bag and sealed to prevent deterioration. 3. Other reagents not used should be packaged or covered. Do not mix reagents of different batches. Use before warranty. 4. Use a disposable tip to avoid cross-contamination. Avoid pipettes with metal parts when drawing stop solution and substrate A and B. 5. Use a clean plastic container to configure the wash solution. Mix all the ingredients and samples in the kit thoroughly before use. 6. Wash the enzyme plate should be fully patted dry, do not put the absorbent paper directly into the enzyme standard reaction well to absorb water. 7. Substrate A should be volatilized to avoid opening the lid for extended periods of time. Substrate B is sensitive to light and avoids prolonged exposure to light. Avoid contact with hands and be toxic. The OD value should be read immediately after the experiment is completed. 8. The order of addition of reagents should be consistent to ensure that all wells are incubated for the same time. 9. The incubation was carried out according to the time indicated in the instructions, the amount of addition and the order. Sample collection, processing and storage methods 1. Serum ---- Avoid any cell irritation during the procedure. Use tubes without pyrogens and endotoxins. After collecting the blood, the serum and red blood cells were quickly and carefully separated by centrifugation at 1000 x g for 10 minutes. 2, plasma ----- EDTA, citrate, heparin plasma can be used for testing. The pellet was removed by centrifugation at 1000 x g for 30 minutes. 3. Cell supernatant - 1000 x g was centrifuged for 10 minutes to remove particles and polymer. 4, tissue homogenate ----- the tissue is added to the appropriate amount of physiological saline mash. Centrifuge at 1000 × g for 10 minutes, take the supernatant 5. Storage ------If the sample is not used immediately, it should be divided into small parts - 70 °C to avoid repeated freezing. Do not use hemolysis or hyperlipemia as much as possible. If there are large amounts of particles in the serum, centrifuge or filter before testing. Do not heat thaw at 37 ° C or higher. It should be thawed at room temperature and ensure that the sample is fully thawed evenly. [Isoproterenol (iso-Hyd) ELISA kit reagent preparation] 1. Standard: The serial dilution of the standard should be prepared during the experiment and cannot be stored. Mix the standard shakes before dilution. The dilution ratio is as follows: 80 ng/ml (No. 6 standard) The original concentration was directly added to 50 ul without dilution. 40 ng/ml (No. 5 standard) Add 100 ul of standard dilution to 100 ul of the original standard 20 ng/ml (No. 4 standard) 100ul of standard 5 is added to 100ul of standard dilution 10 ng/ml (No. 3 standard) 100ul of standard 4 is added to 100ul of standard dilution 5.0 ng/ml (No. 2 standard) 100ul of standard 3 is added to 100ul of standard dilution 2.5 ng/ml (No. 1 standard) 100ul of standard 2 is added to 100ul of standard dilution 0 ng/ml (blank control) The original concentration was directly added to 50 ul without dilution. 2. Dilution of wash buffer (50 x): 50-fold dilution of distilled water. Steps 1. Mix all reagents thoroughly before use. Do not allow the liquid to generate a large amount of foam, so as to avoid adding a large amount of air bubbles during the loading, resulting in errors in the loading. 2. The number of slats required is determined by the number of samples to be tested plus the number of standards. Multiple holes are recommended for each standard and blank hole. Each sample is determined according to its own quantity, and it is possible to use the double hole as much as possible to make a double hole. The specimen was diluted 1:1 with the specimen dilution and 50 ul was added to the reaction well. 3. 50 ul of the diluted standard was added to the reaction well, and 50 ul of the sample to be tested was added to the reaction well. Immediately add 50 ul of biotinylated antibody. Cover the membrane, mix gently by shaking, and incubate for 1 hour at 37 °C. 4. Remove the liquid from the well, fill each well with the washing solution, shake for 30 seconds, remove the washing solution, and pat dry with absorbent paper. Repeat this operation 3 times. If washing with a washer, the number of washings is increased once. 5. 80 ul of affinity streptavidin-HRP was added to each well, and the mixture was gently shaken and incubated at 37 ° C for 30 minutes. 6. Remove the liquid from the well, fill each well with the washing solution, shake for 30 seconds, remove the washing solution, and pat dry with absorbent paper. Repeat this operation 3 times. If washing with a washer, the number of washings is increased once. 7. Add 50 μl of each of the substrates A and B to each well, mix gently by shaking, and incubate at 37 ° C for 10 minutes. Avoid lighting. 8. Remove the microplate and quickly add 50 ul of stop solution. Immediately after adding the stop solution, the results should be determined. 9. The OD value of each well was measured at a wavelength of 450 nm. Recommended experimental protocol Standard concentration (ng/ml) A 80 80 sample sample sample sample sample sample sample sample sample sample B 40 40 sample sample sample sample sample sample sample sample sample sample C 20 20 sample sample sample sample sample sample sample sample sample sample D 10 10 sample sample sample sample sample sample sample sample sample sample E 5.0 5.0 sample sample sample sample sample sample sample sample sample sample F 2.5 2.5 sample sample sample sample sample sample sample sample sample sample G 0 0 sample sample sample sample sample sample sample sample sample sample H sample sample sample sample sample sample sample sample sample sample sample sample Limit The results above the No. 6 standard are non-linear and no accurate results can be obtained from this standard curve. Isoproterenol (iso-Hyd) ELISA kit performance 1. Sensitivity: The minimum detection concentration is less than the No. 1 standard. The linearity of the dilution. The linear regression coefficient of the sample and the expected concentration correlation coefficient R value was 0.990. 2. Specificity: Does not react with other cytokines. 3. Repeatability: The coefficient of variation between the plate and the plate is less than 10%. Analyzing the results of the analysis 1. Instrument value: read the OD value of each hole on a microplate reader with a wavelength of 450 nm. 2. The absorbance OD value is the ordinate (Y), and the corresponding iso-Hyd standard concentration is the abscissa (X), and the corresponding curve is obtained. The iso-Hyd content of the sample can be converted from the standard curve according to the OD value. The corresponding concentration. 3. Detection range: 0-80ng/ml 4. Sensitivity: 0.1 ng/ml Shanghai Fanke Biotechnology specializes in the production and supply of kits: double-anti-sandwich ELISA, ELISA kit, human ELISA kit, enzyme-linked detection kit, domestically imported reagent, elisa kit, ELISA kit, Rat ELISA kit, mouse ELISA kit, apoptosis related factor kit, interleukin ELISA kit, VIP ELISA kit and other laboratory products, product quality, quality assurance. The performance of kit: 1 sensitivity: minimum detection concentration is less than 1 standard. Liiso-Hydarity of dilution. Sample liiso-Hydar regression and the expected concentration correlation coefficient R value is 0.990. 2: no specific reaction with other cytokiiso-Hyds. 3 repeaiso-Hydility: plate, plate between the coefficients of variation were less than 10%. Human type III procollagen amino ring peptide ELISA kit steps: 1 before use, all reagents and mixing. Do not allow liquid to produce a large number of bubbles, so as to avoid adding a large number of bubbles, resulting in the addition of the error. Each of the sample and the hole hole is recommended to do the hole. Each sample can be made according to its own quantity, and can be used as a hole in the hole. 3 diluted after standard 50ul in reaction hole, added to the sample 50 UL in reaction hole to be measured. Immediately joiiso-Hydd the 50 UL antibody biotin. Cover the membraiso-Hyd plate, gently oscillating mixing, 37 degrees Celsius for 45 minutes. 4 left hole liquid, each hole filled with washing liquid, oscillating 30 seconds off the washing liquid, pat dry with absorbent paper. Repeat this operation 4 times. If the washing machiiso-Hyd with washing, washing times increased once. 5 per hole adding chain affinity enzyme -HRP 100ul, gently oscillating mixing, 37 degrees 30 minutes incubation. 6 left hole liquid, each hole filled with washing liquid, oscillating 30 seconds off the washing liquid, pat dry with absorbent paper. Repeat this operation 4 times. If the washing machiiso-Hyd with washing, washing times increased once. 7 per hole adding substrate A, B 50ul, gently oscillating mixing, 37 degrees 5 minutes incubation. Avoid light. 8 remove ELISA plate, quickly add 50ul terminated liquid, adding the stop solution immediately after the determination results. 9 od determination of each hole at the wavelength of 450nm. The result of judgment and analysis: 1, instrument value: Yu Bo 450nm ELISA od read the hole on the instrument 2, to the OD value as a vertical coordinate (y), corresponding ot standard concentrations as a horizontal coordinate (x), do have corresponding curve, sample ot content can be according to the OD value by standard curve conversion out corresponding concentration, multiplied By the dilution multiple; or with the standard concentration and the OD value calculated the regression equation of the standard curve, the sample OD value in the equation to calculate the sample concentration, multiplied by the dilution factor is the actual concentration of the sample. 3, detection range: 0-100ng/ml 4, sensitivity: 0.39ng/ml
How to connect the mobile phone to the PTZ Indoor Camera How to connect the mobile phone to the monitoring camera
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1. Place the camera in position and connect it correctly to the monitoring settings.
2. Since the connection with the mobile phone requires a network, connect the router to the computer. After debugging, insert one network cable into the router and the other into the camera, and then connect the power supply.
3. At this time, turn on the computer, turn on the background of the camera, click [Wireless Function], select [Connect to Wireless Router], and then click [OK] to indicate that the installation is successful.
4. You need to find a network remote monitoring system client corresponding to the mobile phone system and install it on the mobile phone.
5. Log in to the network remote monitoring system client, click the icon [Add Device], enter the user name and password of the home network monitoring camera, and click Save.
6. At this time, you can open [Device List], and its page will display the name of the remote monitoring camera. You need to click the device name, and then click the channel icon above to successfully connect.