At 8 o'clock on the evening of May 25, Xu Mingyan, founder of Shenzhen Haipulos Biotechnology Co., Ltd. shared his experience in the gene sequencing market with his friends. The gene sequencing market is hot, but the upstream sequencers and reagents are basically oligopolistic. Do domestic startups have any opportunities in upstream technologies and products? What should entrepreneurs in the genetic field pay attention to? The following is a wonderful sharing from Xu. This sharing is described in the following six aspects: 1. Various molecular detection technologies Sequencing is based on nucleotides starting at a fixed point, randomly terminating at a particular base, and fluorescent labeling after each base to produce four different sets of ends ending in A, T, C, and G. A series of nucleotides of length are then detected by electrophoresis on a urea-denatured PAGE gel to obtain a visible DNA base sequence. Sanger sequencing is to design primers for mutation sites of known pathogenic genes and perform direct sequencing by PCR amplification. Amplification of a single mutation point (amplification of exon partial fragments including this site) does not require amplification of all exons of the gene in which the point is located. Therefore, single-gene or partial gene-controlled disease samples can be detected, and Sanger sequencing can take advantage of accuracy and low cost. Mutation information is sought by comparing the sequence of the sample to be tested with a known standard sample sequence. One generation of sequencing can only detect a known site in a certain fragment of a gene, the sensitivity is low, and the demand for the sample size to be tested is large. Compared to first-generation sequencing, second-generation sequencing can detect tens to hundreds of genes simultaneously, and can detect information from many unknown sites with a sensitivity of up to 99.99% and a starting sample size of only a few tens of nanograms. The core idea of ​​second-generation sequencing is to synthesize and sequence. Different colors of fluorescent labeling four different dNTPs. When DNA polymerase synthesizes complementary strands, each dNTP will release different fluorescence, according to the captured fluorescent signal. And processed by a specific computer software to obtain sequence information of the DNA to be tested. The high throughput, compared to the detection of a single gene by a generation of sequencing, greatly reduces the cost of sequencing. Amino acids are carboxylic acids containing amino groups. Amino acids are the building blocks of protein for animal nutrition. Proteins in living things are made up of 20 basic amino acids. Alanine for the synthesis of Alitame, intermediate amino acid Serine, nonessential amino acid Arginine Allied Extracts Solutions , https://www.alliedbiosolutions.com
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