Write to your series of four reading literature: mouse, rat euthanasia! ! ! Contributor: Guo Xiaochong Department of Animal Science, China Medical University Write to your series of four reading literature: mouse, rat euthanasia! ! ! When the experimental animal has completed its mission, it should have the right to die without pain. Euthanasia is the product of human civilization, and euthanasia of experimental animals is also a manifestation of civilization. The American Academy of Veterinary Medicine's AVMAGuidelines for the Euthanasia of Animals provides a euthanasia method that can be referenced. (Why don't you use China? Because China's guidelines are still waiting for you to work hard.) The recommended method in the guide is a widely accepted and accepted method, which brings little pain and tension to animals. I will extract the parts that I think are useful as follows. For more information, please refer to the above guide. Recommended mouse and rat euthanasia methods: 1 Excessive anesthesia: intravenous or intraperitoneal injection of 3 times anesthetic dose of barbiturate. 2 cervical dislocation: animals need to be less than 200g. 3 Carbon dioxide asphyxiation: Put the animals in a clean container and slowly introduce carbon dioxide. As the concentration of carbon dioxide increases, the animal will slowly die without pain. Usually 10 minutes is enough to make the animal die, but animals that are euthanized by carbon dioxide asphyxiation must check for complete death, such as the discovery of non-dead animals, supplemented with cervical dislocation. It should be noted that neonatal rats are more tolerant to hypoxia, and the carbon dioxide asphyxiation time needs to be greater than 50 minutes. Finally, I hope that China will pass the euthanasia legislation as soon as possible (human!). I hope that when I am old, I can choose the way to die. Household Clearing Nitrile Gloves Household Clearing Nitrile Gloves,Safety Nitrile Gloves,Oilproof Nitrile Gloves,Household Gloves Prevent Allergic Puyang Linshi Medical Supplies Co., Ltd. , https://www.linshimedical.com