Stem cells are undifferentiated cells with self-renewal and multi-directional differentiation potential. In vivo or in vitro studies have shown that stem cells have phenotypic and functional heterogeneity, which also determines their ability to differentiate and regenerate. There is increasing evidence that the molecular mechanisms of stem cell self-renewal and differentiation and the development of diseases associated with stem cell dysfunction occur at the single-cell level, and the results of heterogeneous stem cell populations are clearly limited. Sex. In recent years, the development of single-cell related technologies has laid an important foundation for the study of heterogeneous cell populations at the single-cell level. The single-cell level of genetic testing has developed rapidly in the past few years. In 2011, there were literatures using single-cell quantitative PCR for gene expression level detection. After the optimization of various gene amplification techniques, many scientists have developed single-cell whole genome sequencing, transcriptome sequencing, and exon sequencing based on high-fidelity gene amplification, further promoting the level of single-cell genes. Expression profiling. However, the various physiological and pathological effects of life medicine, including the differentiation and development of stem cells, ultimately require research and analysis at the protein level to truly resolve the mysteries of these life sciences. In 2014, Professor Amy E. Herr, Department of Bioengineering, University of California, Berkeley, wrote in Nature Methods, Single-cell western blotting, the first time to achieve single-cell precision detection at the protein level, and use this technology to differentiate neural stem cells. The protein expression changes were carefully analyzed. In July 2016, based on this technology, the US company ProteinSimple launched the Milo single cell protein expression quantitative analysis system. The system uses single cell micropore technology to capture single cells, lyse cells in situ, and perform protein electrophoresis separation. Using protein technology for in situ protein capture, specific western blot primary antibody and fluorescently labeled secondary antibody hybridization, after scanning with a chip scanner, Scout software was used to analyze the protein expression in each single cell. From the preparation of the cell suspension to the end of the experiment 4-6 hours, a single cell can perform dozens of target protein analyses. Based on the unique advantages in stem cell heterogeneity analysis, ProteinSimple will bring this technology to participate in the 6th Annual Meeting of Chinese Stem Cells, No. 32, at the Huatian Hotel in Changchun, Jilin Province from September 7th to 9th, 2016. Scientists interested in this technology can meet face-to-face with ProteinSimple's technical experts. Colored adhesive bandage Wenzhou Celecare Medical Instruments Co.,Ltd , https://www.wzcelecare.com