Microbiology testing room The microbiological testing room is also a sterile room, usually a small room in the microbiology laboratory. Can be constructed from sheet and glass. A buffer room should be provided outside the sterile room. The doors of the buffer room and the doors of the clean room should not face in the same direction to prevent the airflow from carrying bacteria. Both the sterile room and the buffer room must be sealed. Ventilation equipment for indoor equipment must have an air filter. The floor and walls in the sterile room must be flat, not easy to hide and dirt, and easy to clean. The table top of the workbench should be level. Ultraviolet lamps are installed in the sterile room and buffer room, and the UV lamp in the sterile room is 1 meter away from the work surface. Workers entering the sterile room should wear sterile clothing and wear a hat. Currently, the sterile room is mostly found in microbiology factories, and the general laboratory uses ultra-clean benches. The main function of the clean bench is to use air laminar flow devices to remove all kinds of tiny dust including microorganisms on the upper surface of the workbench. The electric device passes the high-efficiency filter device through the electric device and enters the work surface, so that the table surface is always under the control of the flow of sterile air. Moreover, on the side close to the outside, there is a high-speed air curtain to prevent external entrained air from entering. Microbiological testing (sterile room) operating procedures ◆ The sterile room should be provided with a sterile operation room and a buffer room. The cleanliness of the aseptic operation room should be 10,000, the indoor temperature should be kept at 20-24 ° C, and the humidity should be maintained at 45-60. %. The cleanliness of the clean bench should reach 100. Wound Dressing,Disposible Wound Dressing,Waterproof Silicone Foam Dressing,Medical Paraffin Dressing Zhende Medical Co.,Ltd , https://www.zhendemedicals.com
The sterile room should be kept clean and no debris should be piled up to prevent contamination.
â—† Strictly prevent all sterilization equipment and culture media from being polluted. Those who have been polluted should stop using it.
â—† The sterile room should be provided with a working concentration of disinfectant, such as 5% cresol solution, 70% alcohol, 0.1% neostigmine solution, and so on.
â—†The sterile room should be sterilized and cleaned regularly with a suitable disinfectant to ensure that the cleanliness of the clean room meets the requirements.
Instruments, instruments, plates, etc. that need to be brought into a sterile room should be tightly packed and sterilized by appropriate methods.
â—† Before entering the sterile room, the staff must wash their hands with soap or disinfectant, then replace the special work clothes, shoes, hats, masks and gloves (or wipe the hands again with 70% ethanol) in the buffer room before entering the sterile The room is operated.
â—† Before use, the sterile room must be sterilized by ultraviolet light from the sterile room for more than 30 minutes, and the ultra-clean table should be opened for blowing. After the operation is completed, the sterile room should be cleaned in time and then sterilized by ultraviolet light for 20 minutes.
â—† Before the test, the outer packaging should be kept intact and should not be opened to prevent pollution. Prior to inspection, the outer surface was disinfected with a 70% alcohol cotton ball.
â—† Negative controls should be performed during each operation to check the reliability of aseptic operation.
â—† When sucking the bacteria liquid, it must be sucked with the ear suction ball. Do not touch the straw directly with the mouth.
â—† Before and after each use of the inoculation needle, it must be sterilized by flame burning. After cooling, the culture can be inoculated.
â—† Pipettes with bacteria liquid, test tubes, petri dishes, etc. should be immersed in a disinfection tank containing 5% of the Sol solution, and taken out after 24 hours.
â—†If the bacteria liquid is sprinkled on the table or on the ground, immediately pour it over the contaminated area with 5% stone carbonate solution or 3% of the sulphur for at least 30 minutes before handling. When the working clothes and caps are contaminated by the bacterial liquid, they should be taken off immediately and washed after autoclaving.
â—† All items with live bacteria must be disinfected before they can be washed under the tap. It is strictly forbidden to pollute the sewer.