The Pharmacopoeia Standard of Scutellaria barbata

Pharmacopeial standards

Pinyin name: Banzhilian

English name: HERBASCUTELLARIAEBARBATAE

The product is a dry whole plant of Lamiaceae Scutellaria barbata D. Don. In the summer and autumn, when the stems and leaves are lush, they are excavated, washed and dried.

Characters: The product is 15-35cm long, hairless or sparse hair on the flower axis. Slim. Stems clustered, thin, square-shaped; surface dark purple or brown green. Leaves opposite, shortly stalked; leaves often shrinking, flattened triangular-ovate or lanceolate, 1.5-3 cm long, 0.5-1 cm wide; apex obtuse, base broadly cuneate, entire or few inconspicuous The blunt teeth; the upper surface is dark green and the lower surface is grayish green. The flowers are solitary in the upper part of the stem, with calyx lobes blunt or round; corolla 2-lipped, brownish-yellow or pale blue-purple, ca. 1.2 cm long, covered with hair. Fruit flat spherical, light brown. Gas slightly, bitter taste. [2]

Determination of content: The preparation of the total flavonoid reference substance solution was accurately weighed and dried at 105 °C to constant weight of scutellarin reference substance 10 mg, placed in a 50 ml volumetric flask, add methanol to the mark, shake, that is (each 1ml Contains Scutellarin 0.2mg).

Preparation of the standard curve precision to take the reference solution 0.4 ml, 0.8 ml, 1.2 ml, 1.6 ml, 2.0 ml, respectively, 25 ml flask, add methanol to the mark, shake. With methanol as a blank, according to the spectrophotometric method (Appendix VB), the absorbance was measured at a wavelength of 335 nm, and the absorbance was plotted on the ordinate and the concentration was plotted on the abscissa.

Accurately measure the amount of wild scutellarin [Content determination] under the Soxhlet extraction and diluted to 100 ml of methanol solution 1ml, set 50ml flask, add methanol to the mark, according to the standard curve preparation method, From the "Methanol blank", the absorbance was determined according to law, and the weight (mg) of the scutellarin in the test solution was read from the standard curve and calculated and obtained.

The product is calculated according to the dry product, and the total flavonoids are not less than 1.50% based on scutellarin (C21H18O12).

Wild baicalin was determined by high performance liquid chromatography (Appendix VI D).

Chromatographic conditions and system suitability test octadecylsilane bonded silica as a filler; methanol - water - acetic acid (35:61:4) as the mobile phase; detection wavelength of 335nm. The number of theoretical plates should not be less than 1500 based on the peak dose of scutellarin.

The preparation of the reference solution was accurately weighed 8 mg of the reference substance of scutellarin and placed in a 100-ml volumetric flask. The mobile phase was added to the scale and shaken. This was obtained (80 μg of scutellarin per 1 ml).

Preparation of the test solution to take the product powder (through the No. 3 sieve) about 1g & # 91; at the same time another product powder to determine the moisture (Appendix IXH first method) & # 93;, accurately weighed, set Soxhlet extractor Add petroleum ether (60-90°C) to a colorless solution, remove the ether solution, remove the petroleum ether from the dregs, continue the extraction with methanol, and transfer to a 100 ml volumetric flask. Add methanol to the mark. Take 25ml, evaporate and the residue is dissolved with 20% methanol and transferred to a 25ml volumetric flask. Dilute to the mark, shake, filter, and continue the filtrate. Filter with a microporous membrane (0.5μm).

The assay accurately draws 10 μl of each of the reference solution and the test solution, and is injected into the liquid chromatograph.

The product is calculated on a dry product, containing scutellarin (C21H18O12) not less than 0.20%. Concocted: Remove impurities, wash, cut into sections.

Taste and origin: Xin, bitter, cold. In the lung, liver, kidney.

Function and Indications: heat detoxification, phlegm and diuretic.

For acne swollen poisoning, sore throat, snake bites, flu or pain, edema, jaundice.

Usage and dosage: 15-30 grams; fresh products 30-60 grams. Appropriate amount of fresh products for external use, apply to the affected area.

Storage: Store in a dry place.

Medication contraindications: blood deficiency is not appropriate, pregnant women careful service.


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